Anthem
Group Companies

Top quality service, timely delivery and proactive communication

Anthem
Group Companies

One stop solution for integrated drug discovery, process development and scale-up

Anthem
Group Companies

Anthem works closely with clients on IP related information

Anthem
Group Companies

The only CRO in the country with Microbiology division that provides antiinfectives screening services

Anthem
Group Companies

Proven Track Record for Hit-Identification, Hit to Lead and lead Optimization

Anthem
Group Companies

Highly qualified scientific team with diverse experience

Anthem
Group Companies

Cloning, expression and purification of recombinant proteins from both bacterial and mammalian host systems.

Assay Biology

The assay biology group at Anthem Biosciences is dedicated to serve our clients perform in vitro biochemical and cellular studies in order to evaluate potency, and other in vitro pharmacology parameters of their compounds. We have developed and customized assays to validate drug targets, as well as screen small molecule drugs for various therapeutic areas including cancer, inflammatory diseases, metabolic disorders, infections (virus, bacterial and fungal), neurodegenerative ailments, and genetic disorders. We have ongoing projects in the development of biologics. We are continuously adding to the list of assays that we can perform. If you do not see the assay that you need below, please get in touch with us to get the current list, or our experts could provide customized solutions for your research needs.

ASSAY BIOLOGY (examples)

Enzymatic assays

  • Ant ioxidants (ORAC)
  • NAD/NADH
  • Nitric oxide synthesis
  • Cox and Lox
  • Prostaglandins
  • Kinase assays
  • Epoxide inhibition assays
  • Hydrolase inhibition assays
  • NF-kb activation assay (reporter based)
  • HDAC assay

Expression and PTM assays

  • mRNA expression (qRT-PCR)
  • Protein expression (Western blot)
  • AMPK activation assay (ELISA)
  • Signal transduction assays (Western blot)
  • Aβ-42 estimation
  • Cytokine, chemokine and growth factors (ELISA)
  • Human APP (ELISA)
  • Target modulation assay for Histone H3 and H4

Cell based assays

  • Cell migration and invasion (trans-well)
  • Wound healing (scratch assay)List item two
  • Tube formation (in vitro angiogenesis)
  • Differentiation assay
  • MTT (cytotoxicity/proliferation)
  • ATP synthesis assay
  • Mitotracker analysis
  • LDH
  • Cell apoptosis (Early detection) – Annexin V
  • Cell apoptosis (Early detection) – Caspase assay
  • PARP cleavage
  • DNA fragmentation
  • Cell cycle analysis (PI staining)
  • Cell cycle regulator expression study (qRT-PCR and Western blot)
  • Cell based reporter assays (promoter and signal transduction pathway analysis)
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