Anthem
Group Companies

Top quality service, timely delivery and proactive communication

Anthem
Group Companies

One stop solution for integrated drug discovery, process development and scale-up

Anthem
Group Companies

Anthem works closely with clients on IP related information

Anthem
Group Companies

The only CRO in the country with Microbiology division that provides antiinfectives screening services

Anthem
Group Companies

Proven Track Record for Hit-Identification, Hit to Lead and lead Optimization

Anthem
Group Companies

Highly qualified scientific team with diverse experience

Anthem
Group Companies

Cloning, expression and purification of recombinant proteins from both bacterial and mammalian host systems.

Drug Metabolism and Pharmacokinetics (DMPK)

The Drug Metabolism and Pharmacokinetics(DMPK) team members possess advanced degrees in relevant specializations from top universities in India and abroad and bring many man years of industrial experience. At Anthem the studies are performed using methods, instrumentation and technologies available with top tier pharmaceutical companies. The studies are carried out in a state of the art facility that includes experimentation labs and a vivarium.

In vitro DMPK

PHYSICOCHEMICAL PROPERTIES

Thermokinetic and thermodynamic solubility (MultiScreen HTS filter plates/ LC-MS/MS method)
Stability in buffer solutions, simulated gastric fluid and simulated intestinal fluid (Fasted and Fed), in plasma of different species like human, monkey, dog, rat and mouse.
Log D(7.4) : Shake flask method (n-octanol/cyclohexane: Phosphate buffer saline)
log P and pKa determination

DISTRIBUTION

Plasma Protein binding determination by Ultrafiltration method and Equilibrium dialysis
Species: human, monkey, dog, rat and mouse
In-vitro tissue binding
Blood cell partitioning
Microsomal binding

ABSORPTION / PERMEABILITY

PAMPA (Parallel Artificial Membrane Permeability Assay)
MDCK-wild type permeability
MDR1-MDCK permeability for P-gp interaction
Caco-2 permeability
a. Unidirectional permeability (Single or Multiple time points)
b. Bidirectional permeability (Single or Multiple time points): with and without Pgp inhibitor
Calcein AM assay for P-gp interaction and inhibition

METABOLISM

Metabolic stability in liver, intestinal microsomes, S9 fractions and hepatocytes
Metabolite identification (phase I and II) and characterization studies using LC- MS/MS
Species: rat, mouse, monkey, dog and human
Hepatocyte stability

In vivo PHARMACOKINETICS

SPECIES

Rat
Mouse
Hamsters
Guinea pigs
Rabbits

TYPES OF PK STUDIES

Single dose
Multiple dose
Cross over
Cassette dose

ROUTES

Oral
Intravenous
Subcutaneous
Intradermal
Intramuscular
Intraperitoneal
Per rectal, etc.

TISSUE DISTRIBUTION STUDIES (ORGANS)

Brain, liver, adipose, skin, muscle, spinal cord, etc.

PHARMACOKINETICS STUDY IN DISEASE MODELS

Cancer models eg. tumor bearing athytymic animals
Diabetes models eg. db / db mouse
Obesity models eg. Ob/ob mouse, DIO mouse
Inflamation models eg. IBD model, arthritis model
Tissue distribution in efficacy models and PK-PD correlation

EXCRETION

Biliary excretion in rats
Renal excretion in rats

PHARMACOKINETICS DATA ANALYSIS

WinNolin (Pharsight)
Parameters reported: Tmax, Cmax, AUClast, AUC∞, Cl, t1/2, Vss, MRT and F%
Plasma to tissue ratio

BIOANALYTICS

Method development and validation
Development of efficient/reproducible extraction procedures (protein precipitation, liquid- liquid extraction, solid-phase extraction)
Recovery and linearity studies
Development of analytical methods in Biological Matrix - plasma, serum, bone marrow, lymph node, urine, feces, bile, other tissues, samples from both in vivo and in vitro studies